Green fluorescent protein and its derivatives as versatile markers for gene expression in living Drosophila melanogaster, plant and mammalian cells. Green fluorescent protein as a marker for gene expression and subcellular localization in budding yeast. K., Riles, L., Johnston, M., & Hegemann, J. TriTag: an integrative tool to correlate chromatin dynamics and gene expression in living cells. Quantitative Imaging of Transcription in Living Drosophila Embryos Links Polymerase Activity to Patterning. Transcriptional Pulsing of a Developmental Gene. Real-Time Observation of Transcription Initiation and Elongation on an Endogenous Yeast Gene. Real-Time Kinetics of Gene Activity in Individual Bacteria. Dynamic visualization of transcription and RNA subcellular localization in zebrafish. Localization of ASH1 mRNA Particles in Living Yeast.
Nature Structural & Molecular Biology, 15(1), 103-105. Structural basis for the coevolution of a viral RNA–protein complex. Imaging of DNA and RNA in Living Eukaryotic Cells to Reveal Spatiotemporal Dynamics of Gene Expression. Distinction and Relationship between Elongation Rate and Processivity of RNA Polymerase II In Vivo. Inference of RNA Polymerase II Transcription Dynamics from Chromatin Immunoprecipitation Time Course Data. Wa Maina, C., Honkela, A., Matarese, F., Grote, K., Stunnenberg, H. Current Protocols in Molecular Biology, 69(1). Chromatin Immunoprecipitation for Determining the Association of Proteins with Specific Genomic Sequences In Vivo. V., Sekinger, E., Yang, A., Moqtaderi, Z., & Struhl, K. Eukaryotic mRNA Decay: Methodologies, Pathways, and Links to Other Stages of Gene Expression. Molecular mechanisms driving transcriptional stress responses. Controlling gene expression in response to stress.
Inducible gene expression: diverse regulatory mechanisms. Encoding and Decoding Cellular Information through Signaling Dynamics. Impulse Control: Temporal Dynamics in Gene Transcription. Eukaryotic transcriptional dynamics: from single molecules to cell populations. Annual Review of Biophysics, 45(1), 25-47. L., Rodriguez, J., Chen, H., & Larson, D. Annual Review of Biophysics and Biomolecular Structure, 36(1), 191-212. Gene Regulation: Gene Control Network in Development. Genomic Expression Programs in the Response of Yeast Cells to Environmental Changes. Diverse and Specific Gene Expression Responses to Stresses in Cultured Human Cells. We will mainly focus on state-of-the-art developments in the yeast model but also cover higher eukaryotic systems.
Tritag michigan update#
Finally, this review will give an update on the mechanisms of heterogeneous gene expression among genetically identical individual cells. Furthermore, we will dissect how cell physiology or age impacts on dynamic gene regulation and especially discuss molecular insights into acquired transcriptional memory. Intrinsic factors modulating gene expression dynamics will then be discussed, focusing on chromatin modifications. We will start by comparing recent in vivo procedures to capture gene expression in real time. In this review, we highlight the dynamic nature of transient gene expression changes to better understand cell physiology and development in general. Changes in gene expression are highly dynamic and depend on many intrinsic and extrinsic factors. The regulation of gene expression is a fundamental process enabling cells to respond to internal and external stimuli or to execute developmental programs.